Agata K Kolodziej-Macczak has her expertise in wound healing and active dressings. She has worked as a Pharmacist, Master degree in Pharmacy and studying medicine as her second Faculty

Rich history of gold that goes back thousands of years and rediscovering various possibilities of use of this metal especially in some medical and pharmaceutical branches focus attention of scientists from all over the world. More and more researches over potential of Gold Nanoparticles (GNPs) in therapies of tumors are being done. Results of experiments comparing properties of different nanoparticles forms and their modifications emerge every year. Maybe it will be possible soon to create a form of gold completely safe as a medicine that could destroy illnesses towards which mankind is still largely helpless. Anyway, gold nanoparticles and some gold complex compounds may have future in oncology ahead. What is more, novel ways GNPs synthesis are being developed, especially those using plants. Some forms of gold are successfully applied in wound healing therapies and regenerative medicine as well as towards various pathogenic microorganisms. Among all kinds of gold forms, high hopes are bound especially to gold nanoparticles as they may be applied in many ways, not only as a remedy, but also used in diagnostic techniques. Gold is recognized as biocompatible material, but more research towards possible side effects is needed. This work presents information set concerning connections between gold applications in different medical fields.

Ryan Bender is currently pursuing his MD at SUNY Downstate Medical Center in Brooklyn, NY and is also working on his research at the Laboratory of Bioregenerative Medicine and Surgery at Weill Cornell Medicine in New York. He has completed his Graduation cum laude from Cornell University with a degree in Biological Engineering and a minor in Biomedical Engineering.

While tissue engineering offers the promise of revolutionary innovation, the cost of bioreactors necessary for large construct tissue culture is often prohibitive. Using our methods, several perfusion-capable tissue-culture devices were built for under 25 USD per device. These devices allowed large 3D tissue culture devices-up to 50 mm×50 mm - to be maintained in a sealed sterile environment after assembly, avoiding the problems associated with unsealed petri dishes. Our devices consisted of individual autoclavable containers with multiple access ports. Media changes were performed via peristaltic pump perfusion or with syringebased cell culture techniques through luer-lock stopcocks. Tissue constructs were live-imaged with lightmicroscopy through the window section of the device and fluorescent-protein-expressing cells were imaged with inverted fluorescence microscopy. The low cost of component parts allows rapid customization for individual experiments, including the addition of separate inlets to adjust media level in compartments of the device. Notably, these devices were maintained on perfusion culture with a pump external to the incubator for 14 days and developed no contamination. While original models of our devices used silicone and parafilm to hold the tubing in place in the lid, newer iterations seek to utilize custom-designed pieces of PDMS that will hold the tubing in place and obviate the use of adhesives for device construction. We hope to make the device even easier to assemble, with quick-locking pieces to streamline assembly.

Agata K Kolodziej-Macczak has her expertise in wound healing and active dressings. She has been connected with the topic for last years while also working as a pharmacist (Master degree in Pharmacy) and studying medicine as her second faculty. Her PhD degree is in progress concerning wound healing and innovative dressings including silver, gold and various natural substances.

Statement of the Problem: Silver is known for its antibacterial properties and has been used in medicine since ancient times. In last year’s many researches proving its effectiveness against wide spectrum of microorganisms have been done and its role in wound healing therapies is still growing. Due to the fact that chronic wounds are huge and severe problem in human population, novel healing methods are assessed all over the world. Nevertheless, amongst all works considering use of silver dressings in medicine, there is not enough information about similarities and differences between various forms of this metal and their pharmacological mechanisms. With reference to this need, following work summarizes medical reports and previous researches about application of silver in wounds. Method: Silver salts, Silver Sulfadiasine (SSD), nano crystalline silver, TIAB molecules and silver ions were taken into consideration. Antimicrobial effects and safety of mentioned forms were assessed as well as influence on healing time. Differences between included researches were precisely analyzed. Findings: Treatment of infected wounds using different forms of silver is mostly satisfactory. Bacteria can hardly develop resistance against silver. SSD and silver salts application must repeated frequently during the day so novel dressings with silver ions or nanocrystals are more desirable therapeutical form but some of them have time limits. The highest risk of body accumulation is in case of nano crystalline silver and SSD. Conclusion: On the basis of numerous researches, it can be said that active dressings including silver ions and TIAB molecules give the best results in wound therapy with little amount of side effects.

Zahra Khodabandeh Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz institute for stem cell and regenerative medicine,Shiraz University of Medical Sciences, Shiraz, Iran.

Statement of the Problem: We hypothesized that the culture of mesenchymal stem cells (MSCs) in enriched scaffold that is made by extracellular matrix of MSCs in 3D culture can improve stem cells differentiation. With regard to these considerations, the current study aimed to fabricate a biological scaffold by culture of Wharton jelly MSCs in 3D alginate scaffold. Method: The human MSCs derived from Wharton‘s jelly were cultured in 3D alginate scaffolds in Dulbecco's Modified Eagle’s Medium (DMEM), supplemented with 10% (v/v) Fetal Bovine Serum (FBS), 10 µg/mL Streptomycin and 100 mg/mL Penicillin for 2 weeks. The cell were made extracellular matrix and added to alginate during 2 weeks. Later decellularization and lyophilization has been done on 3D matrixes. Glycosaminoglycans (GAGs) were identified in extracellular matrix of MSCs by Safarnin o and Alcian blue staining. All the scaffolds were evaluated by Scanning Electron Microscope (SEM) and Fourier Transforms Infrared Spectrometry (FTIR). Scaffold porosity was also determined. The differentiation of MSCs into osteocyte and adipocyte was also compared with control group (cell cultured in 2D culture). Result: FTIR and SEM analysis identified that this scaffold are highly porous with interconnection. This can provide an ideal matrix which is suitable for cell growth and differentiation. The cells were differentiated to osteocyte and adipocyte in this 3D porous scaffolds that can be useful in tissue engineering. Conclusion: The scaffolds showed a different physical and biological property that is useful in tissue engineering applications.