Viral Gene Therapies

Classical methods of gene therapy include transfection. It became inefficient and limited mainly due to delivery of gene into actively proliferating cell s in-vitro. Gene therapy utilizes the delivery of DNA into cells by means of vectors such as biological in-vitro or viral vectors and non-viral methods. The Several kinds of viruses vectors used in gene therapy are retrovirus, adeno-associated virus and herpes simplex virus. While other recombinant viral vector systems have been developed, retroviral vectorsremain the most popular vector system for gene therapy protocols and widest application due to their historical significance as the first vectors developed for efficient gene therapy application and the infancy of the field of gene therapy.

 

  • Vectors for gene therapy
  • Retroviral and other viral vector
  • Non -viral vectors in gene therapy
  • Calcium phosphate transfection
  • Electroporation
  • Transductional targeting
  • Fusion protein targeting

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